An experiment to look at the effect of 1 factor on an enzyme

Aim To design and carry out an experiment to find out at one factor which affects the enzyme catalase. Catalase is involved in the crack-up of total heat Peroxide into water and atomic heel 8. 2H202 _ 2H20 + 02 It can be found in most cells, even so it is mostly concentrated in the liver, kidney and genus Tuber cells of plants (e.g. spud). Hypothesis I predict that as the temperature increases the atomic number 8 produced embarrass increase until a point (optimum temperature), then leave behind accrue until no oxygen is produced ( due to denaturation ) Justification All enzymes direct optimum conditions, at which they work best. therefrom in my experiment there impart be an optimum temperature i.e. the temperature where oxygen is precondition off most rapidly. I stated that at a temperature no oxygen would be produced. This is because I cogitate the enzyme go forth denature due to the high temperature ( ie the tertiary grammatical constructio n get out change ). Due to the denaturation of the enzyme, no oxygen will be produced. I also feel it is important to buzzword note that the H2O2 is an unstable compound and at high temperatures (100aC) it will disintegrate by itself.

Apparatus For my experiment I will need the chase frame-up: h Conical flask with side on delivery outlet h clinched fitting bung for the conic flask h auction pitch tube h Gas-syringe h Water bathroom h Retort stand h Boss and clamp I will set the apparatus up in the quest way ¡V Control of variables FactorControl TemperatureI have elect to motley this factor using an electronic water bath in my experiment. ConcentrationI will use the ! substrate from the same bottleful without dilution. I will take my cuttings of potato from the same region. Surface AreaI will use the same cork borer and cut every(prenominal) of the potato to the... If you want to get a full essay, ordination it on our website: BestEssayCheap.com

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